Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo
윤지혜, 최성현, 고정태, 이빈나, 장훈상, 황인남, 오원만, 황윤찬,
소속 상세정보
윤지혜 ( Yoon Ji-Hye ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
최성현 ( Choi Sung-Hyeon ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
고정태 ( Koh Jeong-Tae ) - Chonnam National University School of Dentistry Department of Pharmacology and Dental Therapeutics
이빈나 ( Lee Bin-Na ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
장훈상 ( Chang Hoon-Sang ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
황인남 ( Hwang In-Nam ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
오원만 ( Oh Won-Mann ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
황윤찬 ( Hwang Yun-Chan ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
Abstract
Objectives: In recent in vitro study, it was reported that osteostatin (OST) has an odontogenic effect and synergistic effect with mineral trioxide aggregate (MTA) in human dental pulp cells. Therefore, the aim of this study was to evaluate whether OST has a synergistic effect with MTA on hard tissue formation in vivo.
Materials and Methods: Thirty-two maxillary molars of Spraque-Dawley rats were used in this study. An occlusal cavity was prepared and the exposed pulps were randomly divided into 3 groups: group 1 (control; ProRoot MTA), group 2 (OST 100 μM + ProRoot MTA), group 3 (OST 10 mM + ProRoot MTA). Exposed pulps were capped with each material and cavities were restored with resin modified glass ionomer. The animals were sacrificed after 4 weeks. All harvested teeth were scanned with micro-computed tomography (CT). The samples were prepared and hard tissue formation was evaluated histologically. For immunohistochemical analysis, the specimens were sectioned and incubated with primary antibodies against dentin sialoprotein (DSP).
Results: In the micro-CT analysis, it is revealed that OST with ProRoot MTA groups showed more mineralized bridge than the control (p < 0.05). In the H&E staining, it is showed that more quantity of the mineralized dentin bridge was formed in the OST with ProRoot MTA group compared to the control (p < 0.05). In all groups, DSP was expressed in newly formed reparative dentin area.
Conclusions: OST can be a supplementary pulp capping material when used with MTA to make synergistic effect in hard tissue formation.
키워드
Direct pulp capping; Mineralization, MTA; Osteostatin
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